Journal: The Journal of toxicological sciences

Article Title: Involvement of P2Y receptors in the protective effect of ATP towards the cell damage in HaCaT cells exposed to H₂O₂.

doi: 10.2131/jts.36.741

Figure Lengend Snippet: Fig. 3. Effect of P2Y receptor inhibitors on ATP- and UTP- induced cytoprotection against H2O2-induced cell damage to HaCaT cells. Cells were incubated with ATP (A) or UTP (B) at a concentration of 10 μM for 6 hr in the presence or absence of MRS2179 (100 μM), MRS2578 (10 μM), NF157 (50 μM), clopidogrel (30 μM), MRS2211 (100 μM), or suramin (100 μM). After incubation, cells were exposed to H2O2 at a dose of 7.5 mM for 6 hr and cell damage was evaluated by means of LDH assay. The data represent the mean ± S.E. (n = 4). ### Indicates that experimental value is significantly different (p < 0.005) compared to the non- H2O2-exposed control group. *, **, and *** Indicate that experimental values are signifi cantly different (p < 0.05, p < 0.01, and p < 0.001, respectively) compared to the H2O2-alone-treated group. § Indicates that exper- imental value is signifi cantly different (p < 0.05) com- pared to the H2O2 plus ATP- or UTP-treated group.

Article Snippet: Suramin, A438079, MRS2179, MRS2578, NF157, clopidogrel hydrochloride and MRS2211 were from Tocris Bioscience (Bristol, UK).

Techniques: Incubation, Concentration Assay, Lactate Dehydrogenase Assay, Control

Journal: Neurobiology of Disease

Article Title: Central P2Y 12 receptor blockade alleviates inflammatory and neuropathic pain and cytokine production in rodents

doi: 10.1016/j.nbd.2014.06.011

Figure Lengend Snippet: Effects of P2Y 12 R antagonists in rat models of inflammatory, neuropathic and acute pain. A, B. Mechanical hyperalgesia (A) and edema (B) before (PRE) after (CFA) intraplantar CFA injection in rats. CFA (100 μl, 50% in saline) was injected intradermally into the plantar surface of the right hind paw. 48 h after treatment with CFA, the extent of edema and mechanical sensitivity were measured on both hind paws. Paw withdrawal threshold values are presented in grams, whereas paw volume values are expressed in ml (mean ± S.E.M.). Injected (CFA), but not control paws demonstrated a significant, 49% decrease of pain threshold and 47% increase in paw volume. *** denotes statistical significance of P < 0.001, Student t test. C, D. Effects of clopidogrel (C) and PSB-0739 i.t. (D) on CFA mediated inflammatory pain behavior 48 h after injection in rats. The graphs show the PWT values in g corresponding to doses indicated on the abscissa. Symbols indicate significant differences from the pre-CFA treatment (**P < 0.01) and post-CFA treatment PWT values ( ## P < 0.01, ### P < 0.001), respectively. One-way ANOVA followed by Neuman–Keuls post-hoc test, n = 6–12/group. E, F. Effects of clopidogrel (E) and PSB-0739 i.t. (F) on neuropathic pain behavior in rats. PWT values were assessed on operated (white triangles) and contralateral hind paws (black triangles) expressed in g. Asterisks indicate significant differences from the postoperative values of saline (SAL) or vehicle (VEH) treated animals (*P < 0.05, **P < 0.01, ***P < 0.001). One-way ANOVA followed by Neuman–Keuls post-hoc test, n = 6–12/group. G, H. Effects of clopidogrel (G) and PSB-0739 i.t. (H) on acute thermal nociception in rats. Y axis values show the change in nocifensive threshold (∆PWT, °C). Animals were treated with P2Y 12 receptor antagonists in doses indicated on the abscissa. Asterisks indicate significant analgesic effect, compared to the pre-treatment values, *P < 0.05, **P < 0.01, paired t -test.

Article Snippet: The following drugs were used: cangrelor (The Medicines Company, Parsippany, NJ USA), clopidogrel hydrochloride (Sanofi-Aventis, Budapest Hungary), 6-OHDA, MLA, MRS2395, reactive blue 2 (all from Sigma-Aldrich), morphine hydrochloride (TEVA, Gödöllő, Hungary), ticlopidine hydrochloride (Tocris), suramin hexasodium salt (Bayer, Wuppertal, Germany), and PSB-0739 (synthesized by Y. Baqi and C.E.

Techniques: Injection, Saline, Control

Journal: Neurobiology of Disease

Article Title: Central P2Y 12 receptor blockade alleviates inflammatory and neuropathic pain and cytokine production in rodents

doi: 10.1016/j.nbd.2014.06.011

Figure Lengend Snippet: Effect P2Y 12 receptor antagonists on mechanical hyperalgesia in inflammatory (CFA) and neuropathic (Seltzer) pain model as well as on acute thermal nociception.

Article Snippet: The following drugs were used: cangrelor (The Medicines Company, Parsippany, NJ USA), clopidogrel hydrochloride (Sanofi-Aventis, Budapest Hungary), 6-OHDA, MLA, MRS2395, reactive blue 2 (all from Sigma-Aldrich), morphine hydrochloride (TEVA, Gödöllő, Hungary), ticlopidine hydrochloride (Tocris), suramin hexasodium salt (Bayer, Wuppertal, Germany), and PSB-0739 (synthesized by Y. Baqi and C.E.

Techniques: Hot Plate Test